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Gauri Dhavan

Chemical shifts (ppm) 

	DNA:			RNA:(1)
	G   A   C   T		G   A   C   U
C1'     86			87-92  
C2'     40			74-76
C3'     80			70-75
C4'     88			80-84
C5'     65			63-69

	G   A   C   T		G   A   C   U
N1	160 213 151 142         146 222 151 142
C2      157 155 159 154         157 151 158 154
N3      146 222 196 160         160 213 196 160
C4      162 158 168 170         153 150 169 169
C5      117 120 99  114         117 120  95 100
C6      154 150 143 140         162 157 138 139
N7      233 230 -   -           233 230 
C8      138 142 -   -           135 138       
N9      170 170 -   -           168 169        
NH2                 -           75  81  98  -

	G   A   C   T		G   A   C   U
H1'     5.8 5.8 5.5 6.0         5.6 6.0 5.6 5.6
H2'     2.6 2.6 2.0 2.1         4.6 4.7 4.5 4.3
H2''    2.7 2.8 2.3 2.6         _   _   _   _    
H3'     4.9 5.0 4.8 4.9         4.5 4.7 4.5 4.6
H4'     4.4 4.4 4.1 4.2         4.4 4.5 4.4 4.3
H5'     4.0 4.2 4.1 -           4.3 4.5 4.4 4.3
H5''    4.1 4.2 4.3 -           4.1 4.2 4.0 4.0

	G   A   C   T		G   A   C   U
P                             -(4.5 4.0 4.0 4.2)

RNA Coupling Constant Ranges (Hz): 

imino (N->H) 	90 
ribose (C->H)	140-180
base (C->H)	180-225
C8->N9		4-8
C8->N7		10
C6->N1		13
C1'->N1		11-13
C1'->N9		11-13
ribose (C->C)	33-45

More specific one bond coupling constants (Hz) 

	   DNA:			RNA: (note: these are conformationally dependent)
	   G   A   C   T	G   A   C   U
C1'-H1'				170-180 
C2'-H2'				155-160 
C2'-H2''                      	---
C3'-H3'				140-150
C4'-H4'				145-155
C5'-H5'				145-150
C5'-H5''			145-150

	   G   A   C   T	G   A   C   U
C8-H8              -   -                -   -
C6-H6      -   -                -   -	
C5-H5      -   -       -        -   -
C2-H2      -       -   -        -       -   -

C1'-C2'				45
C2'-C3'
C3'-C4'
C4'-C5'

	   DNA:		RNA:
Pur:       G   A        G   A
C1'-N9
N9-C8
C8-N7
N7-C5
C5-C6
C6-N1
N1-C2
C2-N3
N3-C4
C4-C5

	   DNA:		RNA:
Pyr:	   C   T        C   T
C1'-N1
N1-C2
C2-N3
N3-C4
C4-C5
C5-N1

Places to Start Reading:

(1) Varani and Tinoco, Quarterly Reviews of Biophysics, 24, 4 (1991) 479-532 This is great review of RNA structure and NMR spectroscopy. You will read it over and over and over.

(2) Wuthrich; NMR of Proteins and Nucleic Acids (1986), John Wiley and Sons The novice must start somewhere, might as well be here. Except for some distance tables not much for the seasoned user.

Last updated May 7th, 1997 (GMD)